bio rad cfx384 touch real time pcr instrument  (Bio-Rad)

Journal: The New Phytologist

Article Title: Defender or accomplice? Dual roles of plant vesicle trafficking in restricting and enabling geminiviral systemic infection

doi: 10.1111/nph.70707

Figure Lengend Snippet: Effect of virus‐induced gene silencing of membrane trafficking‐related genes on tomato yellow leaf curl Sardinia virus (TYLCSaV) accumulation. Relative amount of TYLCSaV DNA in the three most apical leaves of Nicotiana benthamiana plants co‐infected with TYLCSaV and the respective TRV‐derived silencing constructs or empty TRV as control. Viral DNA was measured by quantitative real‐time PCR (qPCR) at 15 d postinfection in silenced (gene indicated between brackets) and nonsilenced (TRV) plants. Values represent the mean from 18 to 28 measurements for silenced plants and 52 for the nonsilenced ones as a negative control for gene silencing. Viral DNA levels were normalized to NbACT and are presented as relative quantities compared with the nonsilenced control (TRV, set to 1). Whiskers were calculated according to the Tukey approach, extending to the most extreme data points within 1.5 × IQR from the lower and upper quartiles. Asterisks indicate samples showing a statistically significant difference with the TRV control (no silencing) according to a Student's t ‐test (****, P ‐value < 0.0001; ***, P ‐value < 0.001; *, P ‐value < 0.05). The experiment was conducted in three independent replicates, yielding consistent results, which are distinguished by dot colours (orange, purple and blue). IQR, interquartile range; TRV, tobacco rattle virus.

Article Snippet: The reactions were carried out in the CFX96 and CFX384 Touch Real‐Time PCR Detection System instrument (Bio‐Rad) following 10 min at 95°C and 40 cycles of 15 s at 95°C and 10 s at 60°C.

Techniques: Virus, Membrane, Infection, Derivative Assay, Construct, Control, Real-time Polymerase Chain Reaction, Negative Control